Neurovalida specializes in using adult human brain tissue microarray to validate CNS drug targets. We offer tailored human brain tissue microarray services that includes, case selection, tissue microarray construction, immunohistochemistry or other assays, image processing and analysis of data from the tissue microarray.
We perform all aspects of data interpretation, metamorph journal development and write comprehensive reports describing the findings from the arrays. Our services include options for further validation. Our aim is to work with you to accelerate drug discovery for CNS disorders. We recognize that each customer will have a different need and we can discuss that need and tailor a solution for you.
What is tissue microarray?
Tissue microarray is a method for studying as many as 60 pieces of brain tissue simultaneously.
The method works by taking a small sample (like a core biopsy) of brain tissue approximately 2 mm in diameter and setting it into a wax block.
The array can contain either many different brain regions or many different brain specimens or a combination of the two.
Once the array is made, thin sections (typically 5-10 um in thickness) can be cut from the array using standard histological techniques.
The section containing 60 brain samples then undergoes immunohistochemistry or a similar process.
Because all of the brain samples are processed on the same glass microscope slide there is no variation in the processing from one case or region to the next and thus reproducibility is very high.
Once stained the slide can be scanned at high resolution and metamorph journals can be used to analyse the data.
The analysis can include things like cell number, cell size, neurite length, neurite number, staining density changes, area of staining etc.
We can then use this data to feed back any changes that are evident with respect to the target of interest.
What is the Brain Bank?
The brain bank was established in 1993 in order to study human neurodegenerative diseases. New Zealanders with and without brain diseases can bequeath their brain to science and their brain tissue is stored in the Brain Bank.
The principal aim of the brain bank is to advance the development of treatment for neurodegenerative diseases. The tissue is stored after being fixed in formalin or as fresh tissue and each brain is dissected according to specific protocols to ensure that precise structures and regions of the brain can be studied. Our Brain Banking protocol has been published in several high ranking journals such as Nature Protocols (Waldvogel et al 2007).