Available Tissue Microarrays
Available tissue microarrays focus on Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and motor neurone disease among others, to provide data on disease-specific markers. For example, our Alzheimer’s disease arrays have data on loads for tau, amyloid, microglia, astrocytes, etc. allowing us to correlate proteins of interest with key pathological features of Alzheimer’s disease. Our brain tissues are also exome sequenced allowing for availability of rich genetic data sets along with Braak, ARP and ApoE scores, in addition to well characterised loads for tau, amyloid, microglia, astrocytes, etc for each tissue microarray. This means that we can correlate proteins of interest with key pathological features of neurodegenerative diseases. We also supply matched neurologically normal controls for each of the neurological conditions and can include a selection of appropriate animal brain cores in each tissue microarray to reference concurrent research activities. We recognize that each customer has different needs and we work towards tailoring a solution that is best for you.
Automated Image Acquisition
We use the VSlide and Olympus SLIDEVIEW VS200 research slide-scanner to acquire images from tissue microarrays and then feed these images into our automated (high content) image analysis program.
Automated Image Analysis
We use MetaMorph® to perform automated high content image analysis which extracts both simple and complex features from the acquired images of the drug target, including cellular and sub-cellular localisation and many other features. Data is interpreted by the Neurovalida research team, who have a combined 60 years of expertise studying the anatomy and chemistry of the human brain.
Electron Microscopy
We use ultrastructural studies to determine where proteins of interest might be in a cell using contrast enhanced methodology, immunostaining methods and gold beads of various sizes for colocalization studies. We provide images and a report of what we find using electron microscopy.