We can localise the target of interest at the anatomical, cellular and sub-cellular level in normal and diseased human brain cores from varied brain regions. Thus, we can validate the drug target in terms of expression levels, map its anatomical localisation in the human brain and determine the cellular type(s) (eg: neurons, astrocytes, pericytes, oligodendrocytes, etc) expressing the target.

We have pre-made tissue microarrays for several brain disorders including Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and Motor neurone disease with control cores for each array. Specifically, we have a pre-made tissue microarray containing the middle temporal gyrus from 29 neurologically normal controls and 29 Alzheimer’s disease donors arrayed on 1 slide. We can also construct customised tissue microarrays per client’s requirement. We use immunohistochemical localisation with target-specific antibodies on arrays, followed by image acquisition and analysis. Antibody specificity can be assessed using blocking peptides, if available, as well as western blotting on human brain tissue. Quantitative Reverse Transcription Polymerase Chain Reaction services for mRNA target validation in human brain are also available if requested to further validate the client’s drug target. In most cases, we provide high resolution digitized images of the drug target in human brain and a detailed report of our analysis by scientists from the research team.